2 guardian spirits you get a little later, I think after buying the right DLC and going to NG+.
As for the shrine in Queen's Eyes... I want to say there is one, but I don't think there is. Just the normal checkpoint ones.
Ah, and when you get to a certain room... You'll know it when you see it. Try to stay out of the room and kite the first enemy. The next one you can lure with a ranged attack. Save your guardian attack thing for the next 2 enemies. After you clear this room it will stay clear.